Host Cell Protein Challenges in Antibody Processing: Insights Into Persistence and Mitigation Strategies.

Authors

Younghoon Oh, Andrew Cooke, Harshal Soni, Susan Liu, Mamadou Bah, Brianna Bellis, Laura Smith, Michael Capaldi, Nicholas Levy, Erik Wilhelm, Kristopher Barnthouse

Published in

Biotechnology and bioengineering. Aug 11, 2026. Epub Aug 11, 2026.

Abstract

Persistence of HCPs remains a major purification challenge in biopharmaceutical manufacturing with direct consequences for product quality, patient safety, and regulatory compliance. Over the last two decades, deeper insights into the mechanisms driving HCP persistence have clarified why certain proteins evade removal during purification. These advances are steering the field away from reactive responses and toward proactive, design-based strategies. This review consolidates current knowledge on the mechanisms underlying HCP persistence with a particular focus on antibody production processes. It examines recent insights into how specific HCP properties contribute to persistence, and how these findings inform the design and assessment of mitigation strategies. Our goal is to provide a comprehensive, yet forward-looking, perspective to help guide future research and development of robust HCP control strategies.

PMID:
42581586
Bibliographic data and abstract were imported from PubMed on 12 Aug 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.