Preparation of magnetic agarose microspheres by spray-chilling method and their application in IgG purification.

Authors

Yuanling Huang, Bianbian Guo, Qiong Xiao, Huifen Weng, Yonghui Zhang, Qiuming Yang, Siyuan Wang, Qian Zhang, Anfeng Xiao

Published in

Carbohydrate polymers. Volume 389. Pages 125647. Oct 01, 2026. Epub Jul 20, 2026.

Abstract

In this study, magnetic agarose microspheres (MAM) were successfully prepared by spray-chilling method. The effects of process parameters such as spray height, aqueous phase flow rate and spray pressure on the morphology and particle size distribution of microspheres were systematically investigated, and the optimal preparation conditions (spray height of 130 cm, aqueous phase flow rate of 0.22 L/min, and spray pressure of 0.25 MPa) were determined. Spherical MAM with uniform particle size distribution was obtained. MAM was characterized by FT-IR, XRD, VSM, etc. The results confirmed that magnetic nanoparticles (MNPs) were successfully embedded in the interior of agarose, and MAM had good superparamagnetism and magnetic responsiveness. SPA-MAM was prepared by coupling MAM with recombinant protein A and used for the purification of IgG in rabbit serum. The results showed that the self-made SPA-MAM had good adsorption effect, and the maximum static adsorption capacity of IgG remained above 95% of the initial adsorption capacity after five adsorption-desorption cycles.

PMID:
42586675
Bibliographic data and abstract were imported from PubMed on 13 Aug 2026.

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