Authors
Takenori Arai, Saki Uehara, Sonoko Kondo, Takehisa Yamamoto, Tomomi Kanazawa, Aiko Yamamoto-Kinami, Kei-Ichi Matsuda, Takashi Matsuzaki, Shinji Yamada, Hirokazu Hikono, Kenji Murakami
Published in
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. Pages 10406387261466243. Aug 13, 2026. Epub Aug 13, 2026.
Abstract
Bovine leukemia virus (BLV; family Retroviridae, taxon species Deltaretrovirus bovleu) infection poses a serious threat to cattle health, and the strategic identification of high proviral-load (PVL) carriers is essential for disease control. We evaluated 2 commercial ELISA kits for the detection of BLV antibodies and indirect identification of high-PVL cattle (EBL ELISA [Nippon Gene] and Leukosis Serum X2 [Idexx]) using a cross-sectional survey of 155 cattle from 17 farms. Based on our quantitative real-time PCR (qPCR) results, we classified tested cattle into non-infected, low-PVL (<400 copies/10 ng DNA), and high-PVL (≥400 copies/10 ng DNA) groups. The 2 ELISA kits had excellent agreement (κ = 0.97; 95% CI [0.93, 1.00]) and moderate correlation (r = 0.617). In a receiver operating characteristic curve analysis, the Leukosis Serum X2 ELISA performed better than the EBL ELISA at identifying high-PVL cattle, with a higher area under the curve (0.840 vs. 0.785) and higher sensitivity (89.7% vs. 84.6%) and specificity (66.4% vs. 56.9%). Both ELISA kits had a moderate ability to distinguish high-level BLV-infected cattle. Our results suggest that a 2-step screening concept could be valuable: ELISA screening followed by confirmatory qPCR testing to identify cattle with a high PVL.
PMID:
42591061
Bibliographic data and abstract were imported from PubMed on 13 Aug 2026.
Read full publication at:
Please sign in
to see all details.
Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 3
- Comments 0