Detection of papillomavirus DNA in uterine flushing samples from healthy mares reveals diverse genotypes with differing host ranges.

Authors

Mariassunta Claudia D'Amato, Anna Cutarelli, Edoardo Vigetti, Tommaso di Palma, Serena Montagnaro, Fabio Del Piero, Michele Napoletano, Giovanna Fusco, Sante Roperto

Published in

Scientific reports. Volume 16. Issue 1. May 08, 2026. Epub May 08, 2026.

Abstract

A molecular study to assess the presence of some papillomaviruses was performed on 105 uterine flushing samples of healthy mares using real time quantitative polymerase chain reaction (qPCR) and digital PCR (dPCR). Bovine, equine, and ovine papillomavirus DNA was detected in 27 uterine flushing samples through dPCR (~ 25.71%); qPCR was able to detect 18 positive samples (~ 17.14%). Differences between the two molecular protocols were significant as shown by McNemar's test (p˂ 0.005). EcPV DNA was detected in 10 samples by dPCR (~ 9.52%) of uterine flushing samples. qPCR detected EcPV DNA in 7 samples (~ 6.66). dPCR revealed OaPV1 DNA in 13 samples (12.38%). qPCR revealed OaPV1 DNA in 9 samples (8.57%). BPV DNA was found by dPCR in 4 samples (~ 3.80%); qPCR revealed BPV DNA in two uterine flushing samples, that is ~ 1.90% of healthy uterine flushing samples. Overall, OaPV1 was the most detected papillomavirus from mare uterus. Similar molecular findings were found in the virobiota of mare vagina. EcPV9 was the most prevalent equine PV genotype. EcPV8, BPV13, ChPV1, ChPV2, OaPV2, OaPV3, and OaPV4 were not detected. Our findings are consistent with non-strict host specificity of BPVs and OaPVs.

PMID:
42103858
Bibliographic data and abstract were imported from PubMed on 14 Aug 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.