[Cloning of Mycoplasma gallisepticum Genome in Saccharomyces cerevisiae Cells].

Authors

D R Kharrasov, G Yu Fisunov, D S Matyushkina, E A Tsoy, E A Semenova, R R Kutukov, I S Rog, D A Sandanova, M M Borodina, V M Govorun

Published in

Molekuliarnaia biologiia. Volume 60. Issue 3. Pages 474-485.

Abstract

To date, the genomes of several mycoplasmas have been cloned in Saccharomyces cerevisiae cells, including Mycoplasma genitalium, M. pneumoniae, and M. mycoides. In this work, the 1-Mb genome of Mycoplasma gallisepticum S6 was cloned in S. cerevisiae. A transposon vector was constructed to deliver a synthetic S. cerevisiae replicon and a selective marker into M. gallisepticum genome. Transplantation of the M. gallisepticum genome with the integrated yeast replicon to yeast cells was performed using two approaches, polyethylene glycol (PEG)-induced transformation of yeast spheroplasts with intact genomic DNA isolated from M. gallisepticum cells and prepared in aragose plugs and PEG-induced fusion of M. gallisepticum cells with yeast spheroplasts. Both of the approaches yielded S. cerevisiae strains with the cloned M. gallisepticum genome, as was verified by nanopore sequencing.

PMID:
42599234
Bibliographic data and abstract were imported from PubMed on 14 Aug 2026.

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