Authors
Eyal Seroussi, Jiarui Cai, Li Ma, Moran Gershoni
Published in
Reproduction (Cambridge, England). Aug 14, 2026. Epub Aug 14, 2026.
Abstract
CoRSIVs are genomic regions with CpG-methylation patterns that differ between individuals, yet are consistent between tissues, within the same individual; therefore, their methylation can be profiled in bodily fluids that are easily obtained, like blood and semen. Bearing in mind the simple epigenetic profiling of CoRSIVs, we tested whether this type of Differentially-Methylated Regions (DMRs) is associated with bovine fertility. Sequence-Read-Archive (SRA) meth BLAST was used to estimate CoRSIVs methylation status in 18 healthy, representative, and age-matched Holstein bulls, among which nine had high (H) sire-conception rate (SCR), and the other nine had low (L) SCR (group averages of SCR: 3.3±0.6 and -3.8±1.8, respectively). This method was also applied to morula and trophoblast SRA methylomes. Analysis with meth BLAST was effective for most (80%) CoRSIVs and showed that CoRSIVs are reprogrammed during blastocyst formation, although this method was incapable of specifically determining the methylation level in CoRSIVs with retrotransposons. In sperm, the effect of global methylation was evident in a common (25%) type of CoRSIVs that is highly (94.5%±4.3%) methylated in sperm. Specifically, a failure to retain hyper-methylation in the sperm plus strand was significantly (p < 0.00025) indicative of low SCR. Comparing global DNA methylation using the latter type of CoRSIVs between sperm and blood can be used as a better biomarker for fertility than using other DMRs with more complex epigenetics.
PMID:
42599243
Bibliographic data and abstract were imported from PubMed on 14 Aug 2026.
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