Authors
Xue Han, Xun Zhou, Jie Liu, Yichong Zou, Yongjia Pang, Rui Li, Xinyu Wang, Bohan Cheng, Zhiping Cao, Peng Luan, Li Leng, ShouZhi Wang, Ning Wang, Hui Li, Yuxiang Wang
Published in
Frontiers in cell and developmental biology. Volume 14. Pages 1896814. Epub Jul 31, 2026.
Abstract
PLIN1 is the predominant structural and regulatory protein associated with lipid droplets (LDs) in avian species and plays an essential role in lipid metabolism. The NCBI RefSeq database currently annotates 10 transcripts at the chicken PLIN1 locus; however, only one transcript has been verified experimentally, while the remaining nine are computational predictions. In this study, we identified five distinct transcript variants encoding four N-terminally divergent protein isoforms of chicken PLIN1 using RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE). Immunolocalization assays showed that all PLIN1 isoforms were targeted to the surface of LDs during preadipocyte differentiation. Functional analyses revealed that PLIN1 isoforms play evolutionarily conserved roles in inhibiting cell proliferation, promoting adipogenic differentiation, and facilitating Fsp27-mediated formation of supersized lipid droplets. Among all isoforms, PLIN1a exhibited the strongest regulatory effects in all functional assays. These findings clarify the transcript diversity and functional differentiation of avian PLIN1, provide new insights into the structural basis of LD regulation, and contribute to a better understanding of lipid storage and metabolic adaptation in vertebrates.
PMID:
42602038
Bibliographic data and abstract were imported from PubMed on 15 Aug 2026.
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