Authors
Dor Simkin, Thomas F Barrett, Lingling Zhang, Michael J Moore, Alissa C Greenwald, Noam Galili, Michael Mints, Salma Ramadan, Victoria Yu, Riley D Z Mullins, Jesse Zaretsky, Porter Bischoff, Douglas Adkins, Wade L Thorstad, Sana D Karam, Randall C Paniello, Jason T Rich, Ryan S Jackson, Patrik Pipkorn, Paul A Zolkind, Guangyong Peng, R Alex Harbison, Rebecca Chernock, Anuraag S Parikh, Sidharth V Puram, Itay Tirosh
Published in
Nature genetics. Aug 14, 2026. Epub Aug 14, 2026.
Abstract
Head and neck squamous cell carcinoma (HNSCC) shows substantial intra- and inter-tumoral heterogeneity. We mapped tumor architecture across HPV-positive and HPV-negative HNSCC through spatial transcriptomics (n = 26). HPV-positive tumors display hypercellularity, higher lymphocyte presence, enriched hypoxia and reduced partial epithelial-to-mesenchymal transition (p-EMT) in malignant cells. We observed two distinct spatial architectures of p-EMT: p-EMT edge, where p-EMT is coupled to fibroblasts at the invasive front via TGFβ, and p-EMT core, in which tumor-infiltrating immunosuppressive macrophages and neutrophils induce p-EMT via oncostatin M in the core of tumor nests. These two p-EMT patterns were consistent across multiple samples from the same tumor, suggesting they are tumor-wide features. Together, these findings reveal that distinct interactions in the tumor microenvironment converge on a similar p-EMT cellular phenotype, but in a different spatial pattern that may have potential biological and clinical implications for our understanding of invasion, immune modulation and new targeted therapeutics for HNSCC.
PMID:
42601456
Bibliographic data and abstract were imported from PubMed on 15 Aug 2026.
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