Authors
Riccardo Pecori, Beatrice Casati, Rona Merdler-Rabinowicz, Netanel Landesman, Khwab Sanghvi, Stefan Zens, Ece Bilgin, Mantiana Topouzi, Kai Kipfstuhl, Veronica Pinamonti, Annette Arnold, Mohammad Abukhalaf, David Gomez-Zepeda, Stefan Tenzer, John M Lindner, Michael Platten, Rienk Offringa, Rafael Carretero, Eytan Ruppin, Erez Y Levanon, Fotini Nina Papavasiliou
Published in
The EMBO journal. Aug 14, 2026. Epub Aug 14, 2026.
Abstract
Neoepitope-based therapies hold great promise for cancer immunotherapy because they target tumor-specific mutations and elicit potent anti-tumor T-cell responses. However, their clinical implementation remains limited by the complexity of neoepitope discovery and uncertainty regarding presentation by tumor cells. A potential alternative is the generation of immunogenic neoepitopes directly within cancer cells through programmable RNA editing. Here, we develop Short Precise-Encodable ADAR Recruiting (SPEAR) gRNAs that harness endogenous ADAR1 to direct precise adenosine-to-inosine (A-to-I) editing at selected transcript sites. Using these gRNAs, we demonstrate the generation of immunogenic neoepitopes through RNA editing at the transcript level, termed editopes. In a proof-of-concept model based on the melanoma antigen MART-1, SPEAR-mediated RNA editing restored antigen-specific T cell recognition and enabled tumor control in vivo. Finally, we developed a computational pipeline to identify candidate tumor-selective neoepitopes across multiple cancer types amenable to guided RNA editing. Our findings establish programmable RNA editing as a strategy for engineering immunogenic editopes and provide a framework for neoepitope-directed cancer immunotherapy.
PMID:
42601452
Bibliographic data and abstract were imported from PubMed on 15 Aug 2026.
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