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Experimental and machine learning analysis of structure-property relationships in GPTMS-crosslinked gelatin scaffolds for bone regeneration.

Created on 16 Aug 2026

Authors

Aria Ghabussi, Nima Azmi, Fereshteh Beheshti, Hamidreza Azimy

Published in

Biomaterials advances. Volume 189. Pages 215112. Aug 11, 2026. Epub Aug 11, 2026.

Abstract

This study develops bioactive freeze-dried porcine gelatin scaffolds crosslinked with glycidoxypropyl trimethoxysilane (GPTMS) and evaluates their suitability for bone-regeneration applications through integrated experimental characterization and mechanism-aware machine learning. Gelatin/GPTMS scaffolds containing 0.9%, 1.2%, and 1.5% GPTMS exhibited interconnected porous architectures, predominantly amorphous hybrid networks, and successful siloxane crosslink formation confirmed by SEM, XRD, and FTIR analyses. The 0.9% GPTMS formulation provided the most favorable mechanical balance, with the highest compressive strength and elastic modulus, whereas higher GPTMS contents increased pore size and accelerated degradation; importantly, GPTMS-containing scaffolds showed apatite-forming ability in simulated body fluid, confirming mineralization capacity absent in pure gelatin. A physics-guided Gaussian Process Regression framework further predicted compressive strength, elastic modulus, and degradation behavior with favorable accuracy within the available dataset, while SHAP-based interpretation identified porosity and GPTMS-derived FTIR features as the dominant mechanistic drivers. Overall, the results demonstrate that GPTMS-crosslinked gelatin scaffolds provide a tunable combination of porosity, mechanical competence, biodegradability, and bioactivity, while mechanism-aware machine learning offers a data-efficient route for scaffold-property prediction and biomaterials design.

PMID:
42603453
Bibliographic data and abstract were imported from PubMed on 16 Aug 2026.

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