Protocol for multimodal programming of murine macrophage phenotypes.

Authors

Gauri Mirji, Sajad Ahmad Bhat, Rahul S Shinde

Published in

STAR protocols. Volume 7. Issue 3. Pages 104767. Aug 18, 2026. Epub Aug 18, 2026.

Abstract

Macrophages continuously sense their environment and adopt distinct functional states in response to danger, repair, and homeostatic signals. Here, we present a protocol for generating murine bone marrow-derived macrophages and programming them with microbial/TLR and nucleic acid-sensing agonists, IL-4, apoptotic cells, and tumor microenvironment analogs. We describe steps for stimulation conditions and downstream immunological assays, including flow cytometry, ELISA, RT-PCR, and multiomics, to comprehensively profile murine macrophage phenotypes and functions across these contexts. For complete details on the use and execution of this protocol, please refer to Mirji et al.¹; Mirji et al.²; and Shinde et al.³.

PMID:
42611700
Bibliographic data and abstract were imported from PubMed on 19 Aug 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.