The isolated Stachel peptide of the adhesion G protein-coupled receptor ADGRG6 is predominantly disordered with local helical propensity.

Authors

Tucker J Shriver, Sandra Berndt, Scott A Robson, Austin D Dixon, Ines Liebscher, Joshua J Ziarek

Published in

Biophysical journal. Aug 19, 2026. Epub Aug 19, 2026.

Abstract

Several members of the adhesion subfamily of G protein-coupled receptors (aGPCRs) are capable of self-activation by an internal agonist sequence (aka the Stachel) that's exposed upon removal or conformational changes of the N-terminal fragment of the receptor. Synthetic peptides derived from the Stachel sequence can be used as exogenous agonists. In the inactive form of the full-length receptor, the Stachel is sequestered as the β13-strand within the GPCR Autoproteolysis-INducing (GAIN) domain, but it engages the seven transmembrane region as a helix when it is either an intramolecular sequence or a synthetic peptide. Little is known about the molecular details underlying this transition, but we hypothesize that a disordered conformation is central to this intermediate state in receptor activation. Despite the primarily helical Stachel AlphaFold3 and PEP-FOLD4 models predicted with high confidence for the entire aGPCR subfamily, computational predictions and biophysical experiments reveal a predominantly disordered conformation in solution. Investigating the ADGRG6 (also known as GPR126) Stachel peptide, circular dichroism (CD) and nuclear magnetic resonance (NMR) experiments reveal a predominantly random coil conformation in aqueous buffer, polar detergent micelles, and zwitterionic lipids. Titration of trifluoroethanol uncovered a two-state equilibrium between an unfolded and helix-containing conformation with NMR localizing a single-turn helix to residues L846-L849. Taken together, these data indicate the ADGRG6 Stachel peptide is primarily disordered with a subset adopting partial helical structures, likely requiring the steric hindrance of the receptor binding pocket to fully induce helix formation in an induced fit mechanism.

PMID:
42619266
Bibliographic data and abstract were imported from PubMed on 20 Aug 2026.

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