Nanopore readable activity probes for ribosomal inactivating protein (RIP) toxins.

Authors

Matthew W Turner, Ashley M Bradley, Kristin D Victry, Bailey G Knight, Rachel E Richardson, Natalie M Winans, Lisa M Bramer, Tyler J Sagendorf, Fanny Chu, Kristin M Engbrecht, Jennifer M Mobberley, David S Wunschel

Published in

Analytical methods : advancing methods and applications. Aug 20, 2026. Epub Aug 20, 2026.

Abstract

Ribosome inactivating proteins (RIPs) such as ricin and abrin depurinate an adenine base in the sarcin/ricin loop in the large ribosomal subunit, leading to inhibtion of protein synthesis and cell death. Here, we demonstrate that RIP toxin activity can be detected via nanopore-based DNA sequencing using synthetic oligonucleotide substrates. This is achieved by monitoring the mismatch proportion at the canonical target sequences incorporated into the synthetic substrate and determining the sequence length distribution throughout the entire substrate sequence. The mismatch proportion increases and sequence length distribution decreases with increasing toxin concentration for both ricin and abrin in buffer as well as in more complex backgrounds such as saliva and nasal secretions.

PMID:
42622626
Bibliographic data and abstract were imported from PubMed on 20 Aug 2026.

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