Protocol for identifying glycosylated RNAs using solid-phase chemical conjugation and glycosidase release.

Authors

Jianbo Deng, Xinyu Miao, Xiaotong Wang, Jiajia Li, Shengye Wen, Zeyang Zhou, Yu Gao, Junhong Jiang, Xiaodong Yang, Shuang Yang

Published in

STAR protocols. Volume 7. Issue 3. Pages 104795. Aug 20, 2026. Epub Aug 20, 2026.

Abstract

RNA glycosylation plays a critical role in cellular signaling. Here, we present a protocol for enriching glycoRNAs from cultured cells and clinical tissues using a solid-phase chemoenzymatic technique. We describe steps for employing galactose oxidase (GAO) to selectively oxidize terminal sugar residues, enabling covalent capture on hydrazide resin, followed by enzymatic release using PNGase F. This scalable and robust assay efficiently enriches N-linked glycoRNAs, facilitating the systematic characterization of disease-specific RNA glycosylation patterns.

PMID:
42623236
Bibliographic data and abstract were imported from PubMed on 21 Aug 2026.

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