Near-Infrared Fluorescent Probe for Ratiometric Tracking Endogenous and Exogenous Lysosomal Hypochlorous Acid.

Authors

Chaodan Xiong, Nuo Li, Qiu-Hong Cheng, Qing Wang, Kun Huang

Published in

Journal of fluorescence. Aug 20, 2026. Epub Aug 20, 2026.

Abstract

Hypochlorous acid (HOCl) serves as a vital molecule in innate immune defense, and fluctuations of lysosomal HOCl levels may be linked to a range of physiological and pathological processes, which make the quantitative detection of lysosomal HOCl a necessity. Herein, we report the design and synthesis of a novel fluorescent probe, PD-OCl, which is constructed by conjugating phenothiazine and dicyanoisophorone moieties and exhibits near-infrared (NIR) emission and large Stokes shifts. Notably, PD-OCl can function as a robust ratiometric fluorescent probe for HOCl detection, exhibiting exceptional selectivity, high sensitivity, and rapid response kinetics. Additionally, the PD-OCl has been successfully applied for monitoring both exogenous and endogenous HOCl in living cells, where it displays excellent lysosomal targeting capability, providing a visualized and ratiometric approach for tracing the variations of subcellular HOCl levels. This work establishes PD-OCl as a promising candidate for investigating lysosomal HOCl levels in LPS-induced oxidative stress state cells.

PMID:
42623007
Bibliographic data and abstract were imported from PubMed on 21 Aug 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.