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Network Cluster Analysis of PPI and Phenotype Ontology for Type 1 Diabetes Mellitus.

Created on 21 Aug 2026

Authors

Davood Zaeifi, Mahnaz Azarnia

Published in

Iranian journal of biotechnology. Volume 22. Issue 1. Pages e3502. Epub Jan 01, 2024.

Abstract

Our knowledge of Type 1 Diabetes Mellitus (T1DM) etiology is incomplete; however, the pathogenesis of the disease includes T-cell-mediated destruction of β-cells.
The present study aimed to investigate the key gene pathways and co-expression networks in T1DM disease.
TIDM-associated genes were identified from 13 databases, enrichment of pathways annotated with functional annotations, and analysis of protein-protein network interactions. Next, functional modules and transcription factor networks were constructed. The analysis of gene co-expression networks was conducted to discover associated pivotal modules.
A total of 172 expressed genes and four variants (SNP) were filtered in the of T1DM disease; pathway enrichment analysis identified key pathways, such as inflammatory bowel disease, type I diabetes mellitus, cytokine-cytokine receptor interaction, Th17 cell differentiation, JAK-STAT signaling pathway, and graft-versus-host disease. A weighted correlation network analysis revealed one module that was strongly correlated with T1DM. Functional annotation revealed that the module was mainly enriched in pathways such as T cell activation, regulation of immune system process, and response to the organic substance. IRF2, IRF4, IRF8, and CDX2 were regulated in the module at a significant level.
The study identified IL-2 as a significant T1DM hotspot and highlighted the role of hub genes and transcription factors in the autoimmune disease, offering potentials for treatment and prevention.

PMID:
38827336
Bibliographic data and abstract were imported from PubMed on 21 Aug 2026.

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