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Testing the morphology- and intuition-based taxonomic hypotheses using integrative approach: unexpected discovery of triplet of sibling species within a widespread Eurasian butterfly, Hyponephele lycaon.

Created on 21 Aug 2026

Authors

Elena A Pazhenkova, Vladimir A Lukhtanov

Published in

Cladistics : the international journal of the Willi Hennig Society. Aug 21, 2026. Epub Aug 21, 2026.

Abstract

In well-studied groups of organisms, the taxonomic landscape can be very complex, with several alternative classifications describing diversity within the same set of populations. For example, three hypotheses have previously been proposed to describe the taxonomy of the common and widespread Eurasian Dusky Meadow Brown butterfly, Hyponephele lycaon, and its close relatives. The morphology-based hypothesis (1) is explicitly based on genital differences between two putative species. The other two hypotheses, the hypothesis of multiple allopatric species (2) and the hypothesis of two sympatric species (3) within the H. lycaon complex, are based on scientific intuition rather than on rigorous data and analyses. Here, using an integrative taxonomic approach, we cannot confirm any of the previous classifications, although elements of each can be found in the suggested new system. As a result, we show that Dusky Meadow Brown butterfly is a triplet of sibling species. Two of them, H. collina (Turkey, Levant, Iran) and H. lycaon (Europe, temperate Asia), are allopatric. The third species, H. libanotica, is widely sympatric with H. collina in Turkey, Israel and Iran. We argue that past taxonomic hypotheses, including those based on implicit evidence, are useful priors for planning sampling strategies and more detailed modern taxonomic analyses.

PMID:
42627046
Bibliographic data and abstract were imported from PubMed on 21 Aug 2026.

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