Authors
Ruilin Fan, Zhenlong Han, Yue Dong, Qinzeng Xu, Sudong Xia, Peiqing He
Published in
Marine genomics. Volume 87. Pages 101268. Sep 30, 2026. Epub Aug 10, 2026.
Abstract
Microbial phytases are crucial to organic phosphorus mineralization in marine phosphorus cycle, however, phytases from deep-sea bacteria remain poorly characterized. Here, we isolated and cultured a phytate-hydrolyzing Winogradskyella sp. 266wxhm from sponge Hymeniacidon sp. collected at a depth of 2435 m in the South Atlantic Ocean. The strain 266wxhm showed the highest similarity of 16S rRNA gene with Winogradskyella poriferorum UST030701-295ᵀ (96.61%), and the average nucleotide identity (ANI) was 76.90% with W. poriferorum UST030701-295ᵀ. These results suggest that strain 266wxhm represents a novel species of Winogradskyella. Whole-genome sequencing revealed a single circular chromosome of 3.96 Mb with a GC content of 33.33%, containing 3572 genes, including 3512 CDSs. Genes targeting phosphate uptake and regulation, organic phosphorus turnover, polyphosphate metabolism, and phosphonate-associated metabolism were identified. Notably, A.266wxhm_GM001357 was identified and annotated as a gene encoding 3-phytase (K01083/EC 3.1.3.8), containing conserved residues associated with Ca2+ coordination and structural stabilization, and belonged to the β-propeller phytase family. Finally, recombinant expression assays of A.266wxhm_GM001357 confirmed the phytase activity. This study provides microbial evidence for phytate-derived phosphorus mineralization in the deep sea.
PMID:
42629110
Bibliographic data and abstract were imported from PubMed on 22 Aug 2026.
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