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Nicotinamide mononucleotide combined glucose counteracts iron overload induced mitochondrial dysfunction in mice under hypobaric hypoxia.

Created on 22 Aug 2026

Authors

Fancheng Tan, Xiaoyun Huang, Xiaoya Mao, Yanyi Wang, Xingyao Wang, Feitai Tang, Xing Chen, Ridong Mao, Xiaoyu Gong, Lin Chen, Li-Jun Di, Shen Zhang, Jun Zeng, Shu-Hai Lin

Published in

Molecular & cellular proteomics : MCP. Pages 101648. Aug 21, 2026. Epub Aug 21, 2026.

Abstract

Extreme high-altitude environment poses severe threats to human health, underscoring the urgent need for effective, safe metabolic interventions. Here, we demonstrate that combined regimen of nicotinamide mononucleotide (NMN) and glucose attenuates tissue injury and prevents body weight loss in mice under hypobaric hypoxia (HH). By leveraging a multi-tissue integrative atlas encompassing metabolome, lipidome, proteome, and phenotypic profiles, we identified HH-induced iron overload and oxidative stress as key pathological drivers. NMN plus glucose supplementation significantly counteracted metabolic disruptions across multiple tissues. Mechanistically, HH induced transferrin-inspired iron delivery, causing iron overload and oxidative stress, along with glutathione depletion and lipid peroxidation across multiple tissues. Notably, we observed no significant changes in the protein levels of ferroptotic markers including ACSL4, GPX4, and FSP1, although cannot rule out the possibility of activity alterations of these proteins. Nevertheless, NMN combined with glucose effectively reversed the ferroptosis-associated metabolic alterations and alleviated mitochondrial dysfunction. Collectively, our study provides a systems-level metabolic atlas and reveals that NMN combined glucose mitigates HH-induced multi-organ injury by suppressing ferroptosis through metabolic reprogramming, offering a therapeutic potential for high-altitude hypoxia.

PMID:
42628841
Bibliographic data and abstract were imported from PubMed on 22 Aug 2026.

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