The hybrid energy metabolism profile of human colorectal cancer cells.

Authors

Rafael Moreno-Sánchez, Emma Verónica Moreno-Saavedra, Emma Saavedra, Jorge Luis Vargas-Navarro, Sara Rodríguez-Enríquez

Published in

Biochimie. Aug 22, 2026. Epub Aug 22, 2026.

Abstract

In a continuous effort to characterize the energy metabolism of colorectal cancer, the glycolytic and oxidative phosphorylation fluxes of human colorectal cancer Caco2 cells, cultured and incubated under various conditions, were assessed. Furthermore, the activities and kinetic parameters of several glycolytic and antioxidant enzymes were determined. Both pathways made similar contributions to the cell ATP supply, indicating a hybrid energy metabolism in these colorectal cancer cells, rather than the widely assumed "glycolytic phenotype" commonly associated with this type of cancer. The kinetic analysis of glycolysis and antioxidant pathway revealed no significant differences in enzyme activities and kinetic parameters among Caco2 cells grown under different conditions. However, (a) the glycolytic LDH/PyK, PyK/PFK-1 and PFK-1/HK, and antioxidant G6PD/IDH and CAT/SOD activity ratios, and (b) the strong HPI inhibition exerted by Ery4P indicated clear regulatory differences between cancer and non-cancer cells, which may be considered as potential metabolic biomarkers and suitable drug targets.

PMID:
42632407
Bibliographic data and abstract were imported from PubMed on 23 Aug 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.