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GAMclust: identification of regulated metabolic modules in bulk, single cell and spatial gene expression data.

Created on 23 Aug 2026

Authors

Anastasiia Gainullina, Evgeniia Chikina, Maxim Artyomov, Alexey Sergushichev

Published in

Bioinformatics (Oxford, England). Aug 22, 2026. Epub Aug 22, 2026.

Abstract

Metabolism operates as a highly interconnected biochemical network, and its regulation emerges from coordinated changes across many reactions and metabolites. The integration of gene expression profiling data with organism-scale metabolic networks has proven to be a valuable tool for understanding cellular metabolic regulation. However, the increasing complexity of profiling technologies and experimental designs requires the development of specialized tools.
Here, we present GAMclust, an R package implementing and extending the previously published GAM-clustering pipeline for identifying transcriptionally regulated metabolic modules in complex gene expression datasets. GAMclust supports bulk, single-cell, and spatial gene expression profiling. It includes built-in KEGG and Rhea metabolic networks for human and mouse, with options to expand these networks for the analysis of other species. The package also offers a suite of post-processing and visualization tools, facilitating the exploration and interpretation of results.
GAMclust is freely available at https://github.com/alserglab/GAMclust and https://doi.org/10.5281/zenodo.21432552 under the MIT license. Documentation is available at https://alserglab.github.io/GAMclust. Source code for supplementary materials is available at https://github.com/alserglab/GAMclust-paper.
Supplementary data are available online.

PMID:
42633560
Bibliographic data and abstract were imported from PubMed on 23 Aug 2026.

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