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Serial sarcomere adaptation to eccentric overload training is blunted in ovariectomized rats.

Created on 24 Aug 2026

Authors

Amelia Rilling, Alexandra Q Kirkup, Avery Hinks, Martino V Franchi, Geoffrey A Power

Published in

Physiological reports. Volume 14. Issue 16. Pages e71070.

Abstract

Eccentric contractions performed to long muscle lengths impose high mechanical stress and strain on the muscle fiber, providing a stimulus for longitudinal growth (i.e., increases in serial sarcomeres number (SSN)). Conventional (CONV) resistance training incorporates eccentric and concentric phases; however, mechanical loading is constrained by the concentric phase. Eccentric overload (ECCOVERLOAD) training has a maximal load throughout the eccentric phase increasing the potential for greater sarcomerogenesis. The purpose was to investigate ECCOVERLOAD and CONV training on mechanical and morphological adaptation in ovary-intact and OVX rats. Intact and OVX Sprague-Dawley rats performed 4 weeks (×3/week) of ECCOVERLOAD or CONV resistance training. Mechanical measures were tested pre- and post-training. Fascicle length and sarcomere length were measured to obtain SSN. Torque increased (p < 0.001) similarly across groups at the shorter muscle (p = 0.075) and greater at the longer muscle lengths (p = 0.002) in OVX rats. In the soleus, there was a training× hormone status× training group (p = 0.028) interaction, such that intact rats showed the greatest improvement in SSN with ECCOVERLOAD (~13%), which was higher than both CONV intact (~3%) and OVX rats in either training condition (ECCOVERLOAD ~1%, CONV ~5%). Therefore, OVX had a blunted adaptation in longitudinal muscle remodeling in response to ECCOVERLOAD training.

PMID:
42634540
Bibliographic data and abstract were imported from PubMed on 24 Aug 2026.

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