Authors
Kun Hu, Hui Liu, Lu Ding, Jiahui Li, Shue Gu, Xu Zhang, Songlin Guo
Published in
The Journal of pharmacology and experimental therapeutics. Pages 104982. Jul 08, 2026. Epub Jul 08, 2026.
Abstract
Intracerebral hemorrhage (ICH) is a severe stroke subtype with high mortality and limited treatment options. This study evaluated the safety and therapeutic potential of human placenta-derived mesenchymal stem cells (PD-MSCs) in acute ICH. PD-MSCs were isolated, characterized, and assessed for tumorigenicity by subcutaneous implantation in nude mice. Acute ICH was induced in rats by collagenase injection, followed by in situ PD-MSC transplantation. Neurological function, brain water content, and hematoma volume were evaluated, and perihematomal biological changes were examined using immunofluorescence staining, ELISA, and transcriptomic sequencing. PD-MSCs displayed typical mesenchymal stem cell characteristics and showed no evidence of tumorigenicity under the present experimental conditions. In ICH rats, PD-MSC transplantation reduced brain edema and hematoma volume and partially improved neurological outcomes compared with those in untreated controls. PD-MSC treatment also modulated astrocytic responses, as reflected by altered glial fibrillary acidic protein expression and astrocyte morphology, and decreased the proinflammatory cytokines interleukin-6 and tumor necrosis factor-α. Transcriptomic analysis indicated that PD-MSCs influenced inflammation-related and immune-regulatory pathways in perihematomal tissues. AKR1B1, SNAI2, and ADGRE5 were identified as candidate genes potentially associated with PD-MSC-mediated responses after ICH. These findings provide preliminary evidence that PD-MSCs are nontumorigenic and may attenuate acute brain injury after ICH, although longer follow-up, expanded behavioral testing, dose optimization, and mechanistic validation are needed to confirm their long-term safety and therapeutic efficacy. SIGNIFICANCE STATEMENT: Acute intracerebral hemorrhage lacks effective therapeutic options, and secondary neuroinflammatory injury contributes substantially to neurological deterioration. This study provides preliminary evidence that placenta-derived mesenchymal stem cells may attenuate acute brain injury and modulate inflammation-associated responses after experimental intracerebral hemorrhage, supporting further investigation of placenta-derived mesenchymal stem cell-based therapeutic strategies for hemorrhagic stroke.
PMID:
42637665
Bibliographic data and abstract were imported from PubMed on 25 Aug 2026.
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