Primary Culture of Hematopoietic Tissue Cells from Procambarus clarkii and Their Susceptibility to WSSV and DIV1.

Authors

Yuxi Liu, Yiyi Su, Feng Han, Feng Yang, Zekun Huang, Fang Li

Published in

Fish & shellfish immunology. Pages 111673. Aug 24, 2026. Epub Aug 24, 2026.

Abstract

The absence of continuous cell lines from crustaceans highlights the urgent need for optimized primary cultures in aquatic pathology and disease research. This study describes the establishment of primary hematopoietic tissue (HPT) cell cultures system from the crayfish Procambarus clarkii. Optimal cell isolation was achieved through digestion with a mixture of collagenase (types I and IV, each at 0.2%) at 30°C for 30 min, followed by maintenance in Leibovitz's L-15 medium supplemented with 2% fetal bovine serum (FBS) at 27°C. The cultures remained viable for up to 5 days maintaining ∼70% viability. Crucially these primary HPT cells were susceptible to infection by both white spot syndrome virus (WSSV) and decapod iridescent virus 1 (DIV1), as evidenced by viral gene expression and genome copies. This method provides an accessible and physiologically relevant in vitro model for investigating virus-host interactions in P. clarkii, thus facilitating the development of antiviral strategies.

PMID:
42637160
Bibliographic data and abstract were imported from PubMed on 25 Aug 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.