Authors
Kan Xiao, Minfang Zhang, Qingqing Lv, Qilong Xu, Hanqiu Gong, Xiaoye Xu, Junjie Guo, Shaokui Chen, Jiangchao Zhao, Yulan Liu
Published in
The Journal of nutrition. Pages 101801. Aug 24, 2026. Epub Aug 24, 2026.
Abstract
Fish oil, rich in EPA and DHA, exerts beneficial effects on gastrointestinal diseases in humans and animals.
This study aimed to investigate whether fish oil attenuated DON-induced intestinal dysfunction and abnormal expression of mitochondrial fission/fusion-related proteins in weaned piglets.
Twenty-four weaned pigs were used in a 2 × 2 factorial design (6 pigs /group) and the factors included dietary treatments (5% corn oil or fish oil) and DON exposure (4 mg/kg DON or not). After 21 d feeding, intestinal samples were collected. IPEC-1 cells were treated with 41 μM EPA or 38 μM DHA, with or without 0.5 μg/mL DON. siRNA targeting mitochondrial fission/fusion-related proteins (dynamin-related protein 1 (Drp1), mitofusin-1 (Mfn1) and mitofusin-2 (Mfn2)) were used to explore the molecular mechanism.
Fish oil improved growth performance (P < 0.05) and increased expression of mitochondrial fission/fusion-related proteins including Drp1, Mfn1, and Mfn2 (P < 0.05), accompanied by restoring jejunal morphology, digestion, and barrier function, and mitochondrial function (P < 0.05) of piglets after DON exposure. EPA or DHA attenuated cell damage, mitochondria dysfunction, cell apoptosis (P < 0.001), and increased Drp1, Mfn1 and Mfn2 protein expression (P < 0.05) after DON exposure in IPEC-1 cells. EPA/DHA alleviated cell injury, mitochondria dysfunction and cell apoptosis (P < 0.001) in the presence of siDrp1 and siMfn1, however EPA or DHA could not alleviate these indexes (P > 0.05) in the presence of siMfn2.
The protective effects of EPA/DHA against DON-induced intestinal injury were strongly associated with Mfn2 signaling.
PMID:
42637136
Bibliographic data and abstract were imported from PubMed on 25 Aug 2026.
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