Molecular mechanism of GTPase-activating protein-assisted hydrolysis of GTP catalyzed by Cdc42.

Authors

Kechen Lu, Yihan Liu, Guanyi Li, Yuxuan Wang, Fengli Li, Ting Shi, Yaxue Zhao

Published in

International journal of biological macromolecules. Pages 154175. Aug 24, 2026. Epub Aug 24, 2026.

Abstract

Cell division control protein 42 homolog (Cdc42), a key regulator of cell proliferation and migration implicated in cancer progression, cycles between active GTP-bound and inactive GDP-bound states. GTPase-activating proteins (GAPs) promote Cdc42 inactivation by accelerating GTP hydrolysis, but the underlying catalytic mechanism remains incompletely understood. Here, integrated molecular dynamics (MD) simulations and density functional theory (DFT) calculations demonstrate that GAP binding promotes hydrolysis through a combination of active-site preorganization, enhanced electrostatic interactions with phosphate, and Arg305-assisted stabilization of the Gln61 position and electrostatics. The Gln61-assisted proton transfer pathway, where Gln61 aligns the water molecule for nucleophilic attack and facilitates proton transfer, is energetically favored over direct proton transfer within the selected preorganized MD conformations. GAP binding is associated with a lower hydrolysis barrier, consistent with GAP-assisted Cdc42 inactivation. These findings elucidate the structural dynamics of the Cdc42-GAP complex and the catalytic role of GAP in GTP hydrolysis. This mechanistic framework can inform future studies of Cdc42 regulation and its relevance to cancer-related processes.

PMID:
42636932
Bibliographic data and abstract were imported from PubMed on 25 Aug 2026.

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