Authors
Vanna M Tran, Jinghui Tao, Caleb J Rux, David Gomez Siu, Miquel Rosas-Salvans, Sophie Dumont
Published in
The Journal of cell biology. Volume 225. Issue 10. Oct 05, 2026. Epub Aug 25, 2026.
Abstract
The mammalian kinetochore connects chromosomes to dynamic spindle microtubules. To remain attached, it must maintain structural integrity under force, but to what extent and how it does so remain unclear. Under spindle forces, we find using super-resolution microscopy that inner (CENP-A) and outer (Hec1) metaphase kinetochores undergo correlated, large-scale (1 µm) deformations along the force axis, suggesting dynamic, relative sliding of parallel protein linkages. Kinetochore shape changes can be asymmetric, with centromere-facing "tails" correlating with hyperstabilized microtubule attachments. Applying microneedle pulling forces, we demonstrate that kinetochores are elastic, stretching under force and relaxing in seconds afterward. Finally, we show that SMC2 depletion results in more variable kinetochore deformations, despite maintained elasticity, and in reduced microtubule attachment stability. Thus, the kinetochore is structurally highly dynamic, requiring a stable centromere base to maintain structure and function under force. We propose a model whereby individual protein linkages are stiff, yet global kinetochore structure is flexible to accommodate different attachment geometries and forces while maintaining function.
PMID:
42640254
Bibliographic data and abstract were imported from PubMed on 25 Aug 2026.
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