Authors
Zi Yang Dong, Lin Xuan Fang, Cong Ying Song, You Cheng Xing, Xue Li, Tao Cai
Published in
Angewandte Chemie (International ed. in English). Pages e8631704. Aug 25, 2026. Epub Aug 25, 2026.
Abstract
Photocatalytic NADH regeneration is limited by poor recovery of the rhodium mediator (Rh*) and enzyme deactivation caused by free Rh* species and photogenerated holes. In this study, we introduce poly(acrylic acid) brushes grafted onto conjugated microporous polymers (CMPs) to anchor Rh* complexes (cPTT-P-Rh*). The negatively charged brushes ensure excellent dispersibility and prevent Rh* leaching via electrostatic interactions. They also serve as a conductive pathway for internal electron transfer from the photocatalyst to the anchored Rh*, which is more efficient than intermolecular transfer. Moreover, the brushes physically shield photogenerated holes, protecting the enzyme from both free Rh* and hole-induced damage. Using a low-power 10 W 460 nm LED light, this system achieves 87.7% NADH regeneration (with exclusive 1,4-NADH production) within 15 min with a turnover frequency (TOF) of 93.3 h-1, comparable to the highest reported values in the literature. Notably, the enzyme retains 76.8% activity after photocatalysis, and circular dichroism confirms no damage to protein secondary structure. The catalyst maintains 61.1% activity after seven cycles. This brush design uniquely integrates immobilization, electron transfer, and protection for efficient photobiocatalysis.
PMID:
42639952
Bibliographic data and abstract were imported from PubMed on 25 Aug 2026.
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