Salvianolic acid B enhances adipose-derived stem cells (ADSCs)-mediated perforator flap survival through antioxidative and anti-apoptotic mechanisms.

Authors

Liqin Yuan, Chunjie He, Kai Yang, Xiang Xiong

Published in

Biochimica et biophysica acta. Molecular cell research. Pages 120214. Aug 26, 2026. Epub Aug 26, 2026.

Abstract

Skin flap transplantation is often complicated by ischemia-reperfusion injury, leading to tissue necrosis. Salvianolic acid B (Sa B), a key component of Salvia miltiorrhiza, possesses known antioxidative and anti-apoptotic properties. This study investigated whether Sa B preconditioning could enhance the regenerative capacity of adipose-derived stem cells (ADSCs) before transplantation for improving flap survival. We assessed the effects of Sa B on ADSCs viability, migration, differentiation, and resilience to oxidative stress in vitro under normal and oxygen-glucose deprivation (OGD) conditions. Sa B significantly enhanced ADSC viability, proliferation, and migration, while reducing intracellular ROS accumulation and activating antioxidant responses. A mouse perforator flap model was subsequently established to evaluated the in vivo therapeutic efficacy of Sa B-preconditioned ADSCs. Sa B significantly improved ADSCs viability, proliferation, and migration, while reducing intracellular ROS and activating antioxidant responses. Sa B also promoted ADSCs differentiation towards an endothelial lineage and enhanced angiogenic potential. Mechanistically, Sa B enhanced Nrf2/HO-1-mediated antioxidative defense and promoted PI3K/AKT signaling, resulting in increased anti-apoptotic protein expression and reduced mitochondrial apoptosis. In vivo, transplantation of Sa B-preconditioned ADSCs markedly improved flap survival, tissue regeneration, angiogenesis, and cellular proliferation, accompanied by enhanced antioxidant and anti-apoptotic responses. In conclusion, our findings identify Sa B as a promising pharmacological preconditioning agent that improves the intrinsic regenerative capacity of ADSCs through coordinated antioxidative and anti-apoptotic mechanisms. This strategy provides a potential approach for optimizing ADSCs-based therapy and improving flap survival in reconstructive surgery.

PMID:
42648648
Bibliographic data and abstract were imported from PubMed on 27 Aug 2026.

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