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Sex-dependent prediction of autism.

Created on 27 Aug 2026

Authors

Catriona J Miller, Theo Portlock, Denis M Nyaga, Justin M O'Sullivan

Published in

Frontiers in genetics. Volume 17. Pages 1799530. Epub Aug 13, 2026.

Abstract

Autism spectrum disorders (ASD) have a global prevalence of 1%, with a male-to- female diagnosis ratio of roughly 4:1. Several models have been developed to predict ASD using genetic information. However, the influence of biological sex on prediction outcomes remains underexplored.
We present an ensemble model to predict ASD, which integrates polygenic risk scores (PRSs), common genetic variants, and ASD risk genes with the MSSNG whole genome sequencing (WGS) dataset.
Following training, our model achieved an accuracy of 0.68, an area under the receiver operating curve (AUROC) of 0.72, and a recall of 0.77 on the test dataset. Notably, common variants contributed more significantly to ASD prediction in males than females (p < 0.001), with accuracies of 0.69 and 0.66, respectively. The 16p11 locus emerged as particularly predictive for females (p < 0.001). Gene enrichment analysis using the Allen Brain Atlas revealed that expression of ASD risk genes that were significant in females were enriched (FWER < 0.05) in the primary somatosensory cortex, inferior parietal cortex, and parietal neocortex during fetal development. By contrast, male ASD risk gene expression was enriched (FWER < 0.05) in the dorsolateral prefrontal cortex and anterior cingulate cortex across developmental stages (fetal to adult).
These findings underscore a sex-dependent role for common genetic variants in the risk of developing ASD. In doing so, they highlight the utility of ensemble models that incorporate common variation and biological sex for ASD prediction.

PMID:
42657396
Bibliographic data and abstract were imported from PubMed on 27 Aug 2026.

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