Protocol for functional characterization of adult hippocampal neural precursor cells under demyelinating injury conditions in mice.

Authors

Shiva Nemati, Soheila Karimi-Abdolrezaee

Published in

STAR protocols. Volume 7. Issue 3. Pages 104803. Aug 26, 2026. Epub Aug 26, 2026.

Abstract

Adult hippocampal neural precursor cells (Hip-NPCs) are highly influenced by brain injury and diseases. Investigating the properties of Hip-NPCs is important for determining their reparative response. Here, we present a protocol for the functional characterization of Hip-NPCs under physiological and demyelinating conditions in adult mice. We describe steps for assessing of Hip-NPC self-renewal, activation state, proliferation, metabolism, and tri-lineage fate commitment. This workflow enables systematic evaluation of how pathological conditions and therapeutic interventions alter Hip-NPC functional states and adaptive responses. For complete details on the use and execution of this protocol, please refer to Nemati et al.¹.

PMID:
42658684
Bibliographic data and abstract were imported from PubMed on 28 Aug 2026.

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