Cryo-EM structures of Cdr1 reveal snapshots of substrate transport and diverse inhibitor recognition.

Authors

Zhen Wang, Shuting Yang, Binyu Zhang, Hengyi Jiang, Yinxia Li, Rongchao Gao, Yulong Wang, Fengying Fan, Lili Dong, Jiaxuan Qiu, Xiurui Li, Yue Zhou, Alastair I H Murchie, Xuekui Yu

Published in

Science advances. Volume 12. Issue 35. Pages eaef7706. Aug 28, 2026. Epub Aug 28, 2026.

Abstract

In Candida albicans-a World Health Organization fungal priority pathogen-overexpression of the adenosine triphosphate (ATP)-binding cassette transporter Cdr1 drives multidrug resistance. We present seven cryo-electron microscopy structures capturing substrate entry and expulsion. An inward-facing transmembrane channel with three on-off substrate binding sites defines a proposed entry pathway for a single substrate molecule. Coordinated ATP binding to both nucleotide-binding domains induces transmembrane domain closure, driving the substrate expulsion; adenosine diphosphate release following ATP hydrolysis resets the transporter to an inward-open conformation, enabling substrate entry for the next translocation cycle. Structures with three structurally diverse inhibitors resolve two distinct binding modes: one occupying all three substrate sites and another specifically binding two extracellular-proximal sites. These findings provide snapshots of the substrate translocation cycle and structural blueprints for antifungal drug design.

PMID:
42664345
Bibliographic data and abstract were imported from PubMed on 29 Aug 2026.

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