Development of a defined three-strain nitrifying microbial community for ammonification and nitrification under organic nitrogen conditions.

Authors

Htun Inzali, Sakuntala Saijai, Wakako Okada, Jun Ogawa, Akinori Ando

Published in

Bioscience, biotechnology, and biochemistry. Aug 29, 2026. Epub Aug 29, 2026.

Abstract

A simplified synthetic nitrifying microbial community capable of sequential ammonification and nitrification under organic conditions was constructed using three defined strains: Bacillus badius NBRC 15713, Nitrosomonas europaea NBRC 14298, and Nitrobacter winogradskyi NBRC 14297. This co-culture achieved complete ammonification and nitrification under selected organic nitrogen conditions, with NO₃⁻-N accumulation of approximately 56 mg-N/L and no residual NO₂⁻-N detected. RT-qPCR analysis showed that changes in the relative abundance of N. europaea and N. winogradskyi were associated with changes in inorganic nitrogen concentrations during cultivation, indicating that the co-culture supported the growth and activity of nitrifying bacteria despite the presence of organic compounds that inhibit nitrification. This defined three-strain community provides a reproducible model for studying sequential microbial nitrogen conversion under organic nitrogen conditions.

PMID:
42668143
Bibliographic data and abstract were imported from PubMed on 30 Aug 2026.

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