Effects and mechanism of sestrin1 protein on osteoarthritis inflammation: A cellular model study.

Authors

Yawei Wu, Xiaoying Liang, Yuyan Yang, Yunfeng Sun

Published in

Irish journal of medical science. Aug 31, 2026. Epub Aug 31, 2026.

Abstract

Knee osteoarthritis is characterized by inflammation, oxidative injury, and degeneration of cartilage. Approximately 30% of people over 45 years old exhibit radiographic signs of knee osteoarthritis, with symptoms present in roughly half of these cases. Novel non-pharmacological interventions play a critical role in the management of knee osteoarthritis. In the present study, an exercise-induced protein, sestrin1, was investigated to clarify the effects and mechanism of sestrin1 protein in osteoarthritis inflammation.
A cellular osteoarthritis model was established using IL-1β-induced ATDC5 cells. The expression level of the sestrin1 protein was examined via a QRT-PCR experiment. Sestrin1 was knocked down in the cellular osteoarthritis model to evaluate its impact on inflammation and the AMPK-mTORC1 signaling pathway. Data were presented as mean ± SD. The t-test and one-way ANOVA were used for the comparison.
Sestrin1 expression was upregulated in cellular osteoarthritis models; TNF-α, IL-18, IL-6, and IL-1β were exacerbated when sestrin1 was silenced; The AMPK-mTORC1-NF-κB signaling axis was involved in this process, with sestrin1 acting as an activator of AMPK.
Sestrin1 was upregulated in a cellular osteoarthritis model and played a critical role in regulating inflammation and lesions, partially through the AMPK-mTORC1-NF-κB pathway.

PMID:
42671752
Bibliographic data and abstract were imported from PubMed on 01 Sep 2026.

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