Authors
Sergio Mato, Sofía Municio, José L Alonso, Elena R Alonso, Iker León
Published in
The journal of physical chemistry letters. Volume 17. Issue 34. Pages 9919-9926. Aug 27, 2026.
Abstract
The stability-reactivity paradox of β-lactam antibiotics remains a fundamental puzzle in medicinal chemistry: how can cephalosporins maintain high antibacterial efficacy despite having reduced ring strain compared to penicillins? Here, we reveal the intrinsic conformational architecture of 7-aminodesacetoxycephalosporanic acid (7-ADCA), the foundational core of cephalosporins, using laser ablation broadband rotational spectroscopy and high-level quantum chemical calculations. In a striking departure from the hydrogen-bond-mediated activation observed in penicillins, we discovered that 7-ADCA can be electronically preactivated by a subtle n → π* orbital interaction between the carboxylic oxygen and the β-lactam carbonyl. This interaction selectively weakens the amide bond without compromising the scaffold's overall stability, offering an evolutionary trade-off superior to the fragile penicillin nucleus. A comprehensive survey of the Protein Data Bank (PDB) confirms that this gas-phase geometry not only is the biologically operative pharmacophore but also is adopted by approximately 70% of enzyme-bound cephalosporins. These findings provide insights into the electronic features of the cephalosporin core and offer a structural template consistent with its bioactivity, potentially aiding in the rational design of next-generation antibiotics through subangstrom orbital tuning.
PMID:
42679203
Bibliographic data and abstract were imported from PubMed on 02 Sep 2026.
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