Exploring a Novel Whole-Ovary Decellularization Technique for Developing Suitable Ovarian Bioscaffolds.

Authors

Jiaxin Jiang, Yangxin Zhang, Anita Touch, Suxiu Sun, You Yang, Xiaohong Li, Zhun Xiao

Published in

Tissue engineering. Part C, Methods. Pages 19373384261484012. Sep 02, 2026. Epub Sep 02, 2026.

Abstract

Artificial ovary construction requires a biocompatible scaffold that mimics native extracellular matrix. This study developed a novel decellularization technique combining physical preconditioning (freeze-thaw cycle, multipoint needling, and 10% NaCl hypertonic solution) with shortened chemical detergent exposure (0.5% sodium dodecyl sulfate, 1% Triton X-100, and 2% sodium deoxycholate) for intact porcine ovaries. Compared to conventional chemical-only decellularization, the novel method better preserved glycosaminoglycan and collagen content, induced smaller changes in tissue elastic modulus, and supported higher granulosa cell fusion density in vitro. After subcutaneous implantation in mice, the novel scaffolds promoted greater cell infiltration and neovascularization, while triggering a lower M1-macrophage response. These results demonstrate that integrating physical steps enhances decellularization efficiency and biocompatibility, offering a promising strategy for generating functional ovarian bioscaffolds.

PMID:
42684009
Bibliographic data and abstract were imported from PubMed on 02 Sep 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.