Authors
Yirou Liang, Jizhuang Luo, Danhua Wang, Yunbo Li, Bangchao Xi, Pengxin Huang, Jiayun Wu, Linlin Liu, Xiaotong Jiang, Fanxiang Bu, Yang Zha, Guangyu Qiu
Published in
ACS sensors. Aug 28, 2026. Epub Aug 28, 2026.
Abstract
Point-of-care bioaerosol analysis remains a critical gap in perioperative exposure assessment, as existing air-monitoring approaches primarily quantify particle burden while providing limited information on aerosol-associated biomolecular composition. Here, we present Surgical Personalized On-site Tracker (SPOT), a portable microfluidic sampling-to-biosensing system with localized surface plasmon resonance (LSPR) biosensing for dual-parameter assessments of proteinaceous aerosol burden and accessible adenosine triphosphate (ATP). The SPOT platform enables on-site detection of total protein as a surrogate for aerosolized biological loads, whereas the ATP channel quantifies accessible ATP after aerosol collection and sample processing without intentional cell lysis. In a clinical study conducted during thoracic surgeries, SPOT detected markedly elevated bioaerosols compared with nonsurgical conditions, with total proteinaceous bioaerosol burden levels reaching 39.93 pg/m3 and accessible ATP concentrations up to 78.97 pmol/m3. Importantly, minimally invasive procedures exhibited a significantly higher ATP-to-protein ratio than open surgeries, i.e., 3.15 vs 2.07 pmol/pg, revealing disproportionally elevated accessible ATP within surgical plumes despite lower total mass. These findings established ATP-to-protein profiling as a novel metric for functional bioaerosol surveillance and demonstrated the potential of SPOT for perioperative air-quality monitoring and biosafety evaluation.
PMID:
42683557
Bibliographic data and abstract were imported from PubMed on 02 Sep 2026.
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