High-intensity interval training via electrical stimulation enhances endurance by preserving sarcoplasmic reticulum Ca²⁺ transients in mouse skeletal muscle.

Authors

Luke D Flewwelling, Masih Jafari, Shahrzad Khajehzadehshoushtar, Takashi Yamada, Christopher G R Perry, Arthur J Cheng

Published in

American journal of physiology. Cell physiology. Sep 03, 2026. Epub Sep 03, 2026.

Abstract

This study investigated the effects of high/low-intensity interval training (HIIT/LIIT) via electrical stimulation (ES) on muscle performance, specifically focusing on fatigue resistance and cytoplasmic free calcium ([Ca²⁺]i) dynamics in mouse skeletal muscle. Thirty 9-week-old female C57BL6 mice underwent four weeks of involuntary IT-ES in hindlimb plantar flexor muscles in vivo, with stimulation frequencies set at either 20 Hz (LIIT) or 100 Hz (HIIT). Our results showed that IT-ES significantly enhanced fatigue resistance, particularly with HIIT, evidenced by improved muscle torque output and better preservation of tetanic [Ca²⁺]i levels during repeated contractions. Additionally, IT-ES led to increases in sarcoplasmic reticulum (SR) Ca²⁺ handling proteins, such as SR Ca²⁺ ATPase 1 (SERCA1) and the ryanodine receptor 1 (RyR1), as well as mitochondrial respiratory complex proteins, indicating enhanced metabolic adaptations. The results suggest that HIIT-ES is an effective intervention for improving muscle function, intracellular Ca²⁺ management, and mitochondrial content, providing a foundation for future consideration in rehabilitation and clinical settings.

PMID:
42691477
Bibliographic data and abstract were imported from PubMed on 04 Sep 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.