Lycopene Alleviates Fatty Acid-Induced Oxidative Stress and Apoptosis in Bovine Mammary Epithelial Cells via the TFEB-Mediated Autophagy Signaling Pathway.

Authors

Xinyuan Sun, Hongdou Jia, Yuanyuan Chen, Yutong Wu, Jiayi Yan, Yue Yu, Jingxin Wang, Wenqi Ding, Li Ma, Qiushi Xu, Juan J Loor, Chuang Xu, Xudong Sun

Published in

Journal of agricultural and food chemistry. Volume 74. Issue 34. Pages 27111-27122. Sep 02, 2026.

Abstract

High concentrations of free fatty acids (FFA) during early lactation cause metabolic stress and oxidative damage in bovine mammary epithelial cells. This study explored the protective mechanism of lycopene (LYC), a natural antioxidant, against FFA-induced injury in the MAC-T cells. We found that 1.2 mM FFA induced oxidative stress, reactive oxygen species (ROS) accumulation, apoptosis, and impaired autophagy. LYC dose-dependently alleviated FFA-induced oxidative damage, restored autophagy flux, upregulated ATG5 autophagy-related 5 (ATG5), LC3-II, and transcription factor EB (TFEB), and downregulated p62, ROS, and apoptosis. Inhibition of autophagy abolished the protective effects of the LYC. Overexpression of TFEB mitigated FFA-induced dysfunction, while TFEB knockdown aggravated cell injury. These results demonstrate that LYC protects mammary epithelial cells from FFA-induced oxidative damage via regulating TFEB-mediated autophagy, suggesting its potential use in alleviating metabolic stress in periparturient dairy cows.

PMID:
42690786
Bibliographic data and abstract were imported from PubMed on 04 Sep 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.