Applications of transposon-insertion sequencing for understanding bacterial physiology.

Authors

Emily C A Goodall, Stineke van Houte

Published in

Microbial genomics. Volume 12. Issue 9.

Abstract

Transposon-insertion sequencing (Tn-seq) couples transposon mutagenesis with next-generation sequencing to identify the transposon insertion site for thousands of mutants in parallel. It is a powerful technology with a myriad of uses beyond the identification of essential genes required for a cell to grow and divide. Tn-seq is particularly useful as a high-throughput method to assign function to function-unknown genes, which have increased steadily with the abundance of newly sequenced bacterial genomes. Tn-seq has now been adapted for use in over 100 bacterial species. Here, we summarize the applications of Tn-seq for querying bacterial physiology and discuss some of the possible applications for the future.

PMID:
42690707
Bibliographic data and abstract were imported from PubMed on 04 Sep 2026.

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