Unveiling an entirety of TFIIH complex comprising Tfb3, Kin28 and Ccl1, and anti-UV and insecticidal activities of Ccl1 in Beauveria bassiana.

Authors

Min Li, Jian-Ru Feng, Sheng-Hua Ying, Ming-Guang Feng

Published in

Pesticide biochemistry and physiology. Volume 223. Pages 107284. Epub Jul 28, 2026.

Abstract

Transcription factor TFIIH complex in budding yeast consists of seven-subunit subcomplex (Rad25-Rad3-Ssl1-Tfb1/2/4/5) and three-subunit CAK kinase subcomplex (Ccl1-Tfb3-Kin28) working in the respective pathways of nucleotide excision repair (NER) and transcription initiation. Here, we report that eleven subunits including two Rad3 paralogs form an inseparable anti-UV TFIIH complex in Beauveria bassiana, an insect-pathogenic fungus prone to solar UV damage. The complex formed through an interaction network of all subunits including Tfb3, Ccl1 and Kin28, and was tied by so far validated protein interaction networks to two photolyases, two photolyase regulators, and several protein complexes with remarkable photoreactivation activities. Among eight subunits studied previously, Rad3B, Rad25, and Tfb5 exhibited higher activities in photorepair-reliant photoreactivation than NER-reliant dark reactivation and pleiotropic effects in B. bassiana. In the present study on Tfb3, Ccl1 and Kin28, only Ccl1 showed anti-UV activity and pleiotropic effects as did Rad3B, Rad25, and Tfb5. Their pleiotropic effects involved similarly the positive regulation of hyphal growth on scant media, multiple stress responses and insecticidal activity via cuticular penetration, and the negative mediation of blastospore production in vitro. The remaining subunits had little anti-UV activity and limited or negligible other effect in B. bassiana. These findings unveil that TFIIH has evolved into an inseparable complex that depends on Rad3B, Rad25, Tfb5 and Ccl1 to recover solar UV damage through photoreactivation and orchestrate the fungal insect-pathogenic lifestyle on the earth surface, and add full knowledge to the fungal TFIIH complex distinguished from the yeast counterpart comprising two functionally different subcomplexes.

PMID:
42697653
Bibliographic data and abstract were imported from PubMed on 05 Sep 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.