Authors
Nicole M Michaud, Mark R Ten Haaf, Nathan A Crowder
Published in
Journal of neuroscience methods. Pages 110897. Sep 05, 2026. Epub Sep 05, 2026.
Abstract
Visual neuroscience research has adopted the mouse as one of its primary animal models. Collection of perceptually driven behavior in these mice often involves head fixation to present the visual stimulus under controlled conditions and water restriction to motivate responses. However, both head fixation and water restriction can induce physiological stress.
Here, we adapted a popular open-source two-alternative-forced-choice (2AFC) visual psychophysics apparatus to allow unrestrained mice to maintain consistent stimulus viewing position while performing trials for hedonic reward. The stereotyped head positioning further enabled wireless power transfer to implanted LEDs, permitting transcranial optogenetic manipulation of targeted interneuron subtypes in visual cortex during behavior.
Unrestrained mice learned a 2AFC contrast detection task, and required an average of 22.4 days to reach criterion accuracy. Mice showed strong task engagement with hedonic reward, and produced high quality psychometric functions based on thousands of trials.
Task acquisition times and accuracy were comparable to published head-fixed paradigms. Cortical illumination modulated contrast detection in a manner consistent with previous findings.
This approach provides a less stressful and experimentally accessible alternative to head-fixation, lowering technical barriers while preserving the precision required for visual psychophysics.
PMID:
42700892
Bibliographic data and abstract were imported from PubMed on 06 Sep 2026.
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