Physicochemical and in vitro characterisation of antifungal PVA/chitosan composite wet-spun fibres loaded with hydroxypropyl-γ-cyclodextrin/amphotericin B complexes.

Authors

Rahmat Setiady Tasman, Wildan Khairi Muhtadi, Destria Indah Sari, Nur Aisyiah, Andi Amelia Khumaera, Yuyun Suryati Mansur, Andi Mitha Hardiyanti, Mesakh Diki Saputra, Sartini, Achmad Himawan

Published in

International journal of pharmaceutics. Pages 127372. Sep 05, 2026. Epub Sep 05, 2026.

Abstract

Chronic wounds infected by Candida albicans impose a significant clinical burden, yet topical amphotericin B (AMB) delivery is constrained by low aqueous solubility. Wet-spun poly(vinyl alcohol) (PVA)/chitosan fibres loaded with an AMB/hydroxypropyl-γ-cyclodextrin (HP-γ-CD) inclusion complex were developed as a sustained-release antifungal wound dressing. Phase solubility analysis confirmed inclusion complex formation with an apparent stability constant of 36.1 × 10³ M⁻¹ in distilled water (AL-type profile, R² = 0.9984), characterised by Fourier-transform infrared spectroscopy (FTIR), ¹H NMR, and scanning electron microscopy (SEM). IC-SE-1 achieved ∼ 354-fold and ∼ 136-fold solubility enhancement in distilled water and phosphate-buffered saline (PBS, pH 7.4), respectively, while fully retaining antifungal activity (MIC = 1 µg/mL; p = 0.112 vs. pure AMB). The complex was incorporated into fibres at four PVA:chitosan ratios and crosslinked with tripolyphosphate and glutaraldehyde. Formulation P6C4 (PVA 60%:chitosan 40%) demonstrated the most favourable overall performance, with the highest water-holding capacity (346.51 ± 4.10%), swelling (463.40 ± 11.12%), tensile strength (18.47 ± 2.18 MPa), and elongation (431.57 ± 52.22%). Woven fibres maintained inhibition zones against C. albicans from day 2 to day 7, with AMB release exceeding the MIC throughout, while haemolysis remained below 5% (ASTM F756-08). HP-γ-CD complexation enables sustained above-MIC AMB delivery from a dual-crosslinked biopolymeric dressing, providing in vitro proof-of-concept for Candida-infected chronic wounds, pending cytotoxicity and in vivo validation.

PMID:
42700906
Bibliographic data and abstract were imported from PubMed on 06 Sep 2026.

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