A rapid two-step protocol for isolation of DNA-free, high-integrity RNA from gram-negative Bacteria.

Authors

Ravi Chauhan, Varsha Kaushik, Aakruti Patel, Seema Rawat

Published in

Journal of microbiological methods. Pages 107693. Sep 05, 2026. Epub Sep 05, 2026.

Abstract

A two-step protocol employing Tris-equilibrated phenol (pH 4.5) and sodium dodecyl sulfate was optimized to efficiently isolate DNA-free, intact RNA from gram-negative bacteria. This method reduced processing time and reagent use, thereby minimizing RNA degradation and contamination. The extracted RNA was suitable for gene expression profiling.

PMID:
42700847
Bibliographic data and abstract were imported from PubMed on 06 Sep 2026.

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