Discovery of Emodin as a novel competitive KPC-2 inhibitor through virtual screening: Synergistic efficacy with Meropenem against Carbapenem-resistant Klebsiella pneumoniae infection.

Authors

Yan Wang, Jiamin Geng, Bingyan Li, Xuming Deng, Jiaxi Wang, Na Guo

Published in

Bioorganic chemistry. Volume 182. Pages 110461. Sep 02, 2026. Epub Sep 02, 2026.

Abstract

Carbapenem-resistant Klebsiella pneumoniae produces the carbapenemase KPC-2, which rapidly confers resistance and poses a significant threat to public health worldwide. Natural compounds have broad application potential, novel structures, and are not easily recognized by existing bacterial resistance mechanisms. Therefore, through virtual screening based on the KPC-2 protein structure and in vitro antimicrobial assessment, the natural anthraquinone compound emodin was identified as a KPC-2 inhibitor (IC₅₀ = 24.4 μM) and a synergist of meropenem. Molecular dynamics simulation and interaction analyses indicated that emodin competitively occupied the active site of KPC-2 at Trp105 and Thr237, resulting in reduced α-helices and increased β-sheets, thereby inhibiting KPC-2 activity. The combination of emodin and MEM exhibited a synergistic effect. Furthermore, emodin modulates succinate dehydrogenase (SDH) activity and its transcriptional expression, resulting in metabolic perturbation that renders bacteria susceptible to meropenem, thereby potentiating the synergistic antibacterial activity of the combination. This disrupted the metabolic homeostasis of the bacteria, thereby compromising the integrity of bacterial cells and biofilms. No resistance development was observed over 30 generations of passage. In a mouse model of pneumonia, the combination therapy significantly reduced MEM usage, decreased the pulmonary bacterial load by 1.43 log CFU/g of model group, attenuated lung inflammation, and restored normal lung histology. These findings suggest that emodin acts as a promising candidate of KPC-2 inhibitor, which is capable of reinstating carbapenem efficacy against carbapenem-resistant Gram-negative pathogens in vitro and in vivo.

PMID:
42702130
Bibliographic data and abstract were imported from PubMed on 07 Sep 2026.

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