Purification of a Au tracer from a Pt target using UTEVA resin column chromatography.

Authors

Takuya Yokokita, Yudai Shigekawa, Akihiro Nambu, Michiko Kitagawa, Hiromitsu Haba

Published in

Journal of chromatography. A. Volume 1787. Pages 467399. Aug 27, 2026. Epub Aug 27, 2026.

Abstract

Radioactive ¹⁹⁵Au is useful for the long-term biodistribution tracking of gold nanoparticles in cancer diagnostics and therapeutics. This study develops a highly selective separation method for ¹⁹⁵Au produced via the charged particle irradiation of a Pt target using UTEVA resin column chromatography and elucidates the underlying separation mechanism. To optimize the chromatographic conditions, batch experiments were performed with the UTEVA resin in HCl and HNO₃ media using tracer amounts of ¹⁹⁵Au and macro amounts of Pt. ¹⁹⁵Au exhibited high distribution coefficients in HCl, whereas Pt showed negligible adsorption. In HNO₃ media, the distribution coefficients of ¹⁹⁵Au decreased with increasing acid concentration. Subsequent two-step column chromatography using the UTEVA resin achieved a chemical yield of 91.6% and a decontamination factor of 4.6 × 10³ for Pt. To understand this selectivity, density functional theory (DFT) calculations were performed on the interactions between the anionic chloride complexes of Au, Pt, and Ir (a by-product of the nuclear reaction) and the protonated phosphonate cation. The calculations revealed that the Gibbs free energy change for ion-pair formation follows the order of Au < Ir < Pt, suggesting that anionic chloride complexes of Au readily form ion-pair complexes and are extracted. This extraction of Au is driven by a lower dehydration energy and reduced steric hindrance of [AuCl₄]^- compared to the highly charged and octahedral [PtCl₆]^2- and [IrCl₆]^2- ions. This study demonstrates that the UTEVA resin is highly effective for the separation and purification of ¹⁹⁵Au from irradiated Pt targets. The purified ¹⁹⁵Au obtained by this method can be utilized in future research on Au nanoparticles.

PMID:
42702114
Bibliographic data and abstract were imported from PubMed on 07 Sep 2026.

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