Establishment of a chemoradio-resistance colorectal cancer cell model and its changes in biological behavior.

Authors

Shan Liu, Zeng Zhang, Qian An, Yue Jia, Qin Su, Dezhi Zhu, Likun Luan, Hongying Yang, Zhirui Chuan, Xinyuan Liu, Siyu Wang, Yanyan Liu, Fenglian Fu, Qiong Sun, Luyao Ma, Juanrong Yang, Yiming Zhang, Mingzhi Duan, Peijun Yao, Changqing Yang, Yiming Xing, Hongmei Shen, Yongxin Jiang

Published in

Journal of gastrointestinal oncology. Volume 17. Issue 4. Pages 241. Aug 31, 2026. Epub Jun 24, 2026.

Abstract

Colorectal cancer (CRC) is the most prevalent and malignant gastrointestinal tract tumor. The incidence remains high, and the issue of resistance to radiotherapy and chemotherapy is prominent. The aims were therefore to establish a model for radio- and chemoresistance in CRC, elucidating the underlying mechanism that could help identify measures to reverse its resistance and improve the clinical efficacy of radiotherapy and chemotherapy.
A HCT116 and HT29 cell model of radiotherapy and chemotherapy resistance in CRC was established by simulating clinical high-dose shock therapy as a key in vitro method. For the in vivo animal studies, healthy 6-week-old BALB/c nude mice (both genders) were used. The changes in its biological behavior were studied to provide a theoretical basis for improving the efficacy of radiotherapy and chemotherapy in clinical practice.
We successfully established a CRC cell line with radio- and chemoresistance, and it exhibited stronger potential for cell proliferation, colony formation, invasion, and migration than its parental cell line. Furthermore, in nude mouse models, the resistant cells showed stronger tumorigenicity and stronger liver and lung metastasis ability than the parental cells.
This research helps elucidate the molecular mechanism of chemo- and radiotherapy resistance in CRC and can potentially improve the molecular diagnosis and gene therapy of CRC.

PMID:
42703424
Bibliographic data and abstract were imported from PubMed on 07 Sep 2026.

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