A fluorescent aptamer-based method for kanamycin detection incorporating G-quadruplex DNAzyme and exonuclease III-assisted signal amplification.

Authors

Chengyi Hong, Xiaomei Liu, Yuanyuan Zhang, Mengru Dai, Hongfen Yang, Dan Yang, Ren Cai

Published in

Chemical communications (Cambridge, England). Sep 07, 2026. Epub Sep 07, 2026.

Abstract

A fluorescent aptamer sensor for kanamycin was constructed based on G-quadruplex DNAzyme and Exo III-assisted signal amplification. Target binding triggered cDNA release, activating Exo III to cleave HP and release G-quadruplex, which formed a DNAzyme with hemin to catalyze OPD oxidation and generate fluorescence. The method achieved a detection limit as low as 0.063 nM.

PMID:
42704348
Bibliographic data and abstract were imported from PubMed on 07 Sep 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.