Complementation of a human disease phenotype by intercellular mRNA transfer.

Authors

Gal Haimovich, Sandipan Dasgupta, Anand Govindan Ravi, Jeffrey E Gerst

Published in

Cell reports. Volume 45. Issue 9. Pages 117959. Sep 07, 2026. Epub Sep 07, 2026.

Abstract

There is growing evidence that mRNAs undergo intercellular transfer through cytoplasmic connections called tunneling nanotubes (TNTs), but whether transferred mRNAs are translated and affect cellular changes post-transfer is unknown. Using multiple lines of evidence, we show that transferred mRNAs undergo translation and can complement the phenotype of genetic mutations in vitro. For example, the human peroxisome biogenesis disorder, Zellweger Syndrome, results from mutations in genes such as PEX5 and PEX6. We demonstrate that the co-culture of patient-derived PEX6 mutant fibroblasts or PEX5 knockout cells with wild-type cells leads to de novo peroxisome biogenesis. We provide additional examples of genetic complementation via transfer of mRNAs encoding the HSF1 transcription factor or CRE recombinase. Complementation occurs by TNT-mediated mRNA transfer and translation in acceptor cells, but not by exosomes, nor by protein or peroxisome transfer. Our study provides evidence for the physiological significance of mRNA transfer and suggests another approach for mRNA therapeutics.

PMID:
42704713
Bibliographic data and abstract were imported from PubMed on 08 Sep 2026.

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