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FXF-OMe Is a Stable Tripeptide Motif for Ultra-Short Peptide Coacervates That Establishes Structure-Property-Function Relationships.

Created on 08 Sep 2026

Authors

Samuel Hamroff, Lucas Caire da Silva

Published in

Small (Weinheim an der Bergstrasse, Germany). Pages e75668. Sep 07, 2026. Epub Sep 07, 2026.

Abstract

Ultra-short peptide coacervates (USPCs), formed via liquid-liquid phase separation (LLPS) of peptides containing five or fewer amino acids, offer a minimal platform for microreactors and for probing structure-property relationships governing LLPS and liquid-to-solid transitions (LSTs). USPCs form uniquely hydrophobic liquid interiors compared to larger peptide-based and complex coacervates, enabling aqueous reactivity with hydrophobic substrates. As a result, USPCs have emerged as promising candidates for next-generation microreactors with the potential to surpass nanoparticle-based systems in modularity, scalability, and biocompatibility. Existing USPCs face two major challenges: poor stability against LSTs, which irreversibly eliminate the liquid microenvironment, and a lack of well-developed structure-property relationships that would enable rational materials design. Here, we establish FXF-OMe as a minimal motif, inspired by previously reported sticker-spacer USPC motifs, that dramatically enhances resistance to LSTs while enabling investigation of structure-property relationships. Using this platform, we identify hydrophobic effects as the dominant determinant of coacervate material properties over specific peptide-peptide interactions. We further demonstrate that improved stability and tunability enable selective dye partitioning, enhanced reaction yields, and kinetic control in sulfur photo-oxidation reactions. Collectively, these results position FXF-OMe as a foundation for de novo USPC design and establish USPCs as tunable, stable platforms for precision microreactor applications.

PMID:
42706838
Bibliographic data and abstract were imported from PubMed on 08 Sep 2026.

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